When performing spectral binning in Profiler, there are two normalization methods available, total area and standardized area. Which one should I use?
8 February 2016 Comments Off on When performing spectral binning in Profiler, there are two normalization methods available, total area and standardized area. Which one should I use? FAQ - Profiling Spectra
You should select a normalization method based on the nature of the dataset that you are binning. Use total area to reduce the influence of dilution effects among the samples in your dataset; this is the most common scenario. If you can assume that dilution effects are insignificant, use standardized area.
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