I can change the CSI line width when processing spectra in Processor. Can I similarly change the line width of the signature line in Profiler, setting different line widths for different compounds to get better fits?
8 February 2016 Comments Off on I can change the CSI line width when processing spectra in Processor. Can I similarly change the line width of the signature line in Profiler, setting different line widths for different compounds to get better fits? FAQ - Profiling Spectra
In short, no, you cannot change the line width of the signature line in Profiler.
The line width of the signature lines in Profiler is directly calculated from the line width of the CSI that you set in Processor, and is applied equally to every compound that you fit in Profiler. Thus, it is not possible to set different line widths for different compounds.
You may get better results with a different CSI (we recommend DSS), or if you are working with samples that have some protein content, you may want to try filtering your samples using a 3kDa molecular weight cutoff filter to remove the proteins. Also, some variation in line widths may occur due to varying shimming technique during data acquisition, especially if more than one person is involved in acquiring the spectra.
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